human plasma samples from patients and normal individuals Search Results


plasma from hiv-1 + /hcv − /hbv − individuals ( n = 10) and hiv − / hcv − /hbv − controls ( n = 10)  (BioIVT Inc)
90
BioIVT Inc plasma from hiv-1 + /hcv − /hbv − individuals ( n = 10) and hiv − / hcv − /hbv − controls ( n = 10)
Nef expression and <t>HIV-1</t> infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.
Plasma From Hiv 1 + /Hcv − /Hbv − Individuals ( N = 10) And Hiv − / Hcv − /Hbv − Controls ( N = 10), supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasma from hiv-1 + /hcv − /hbv − individuals ( n = 10) and hiv − / hcv − /hbv − controls ( n = 10)/product/BioIVT Inc
Average 90 stars, based on 1 article reviews
plasma from hiv-1 + /hcv − /hbv − individuals ( n = 10) and hiv − / hcv − /hbv − controls ( n = 10) - by Bioz Stars, 2026-06
90/100 stars
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individual human semen and plasma from normal healthy donors  (BioIVT Inc)
90
BioIVT Inc individual human semen and plasma from normal healthy donors
Nef expression and <t>HIV-1</t> infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.
Individual Human Semen And Plasma From Normal Healthy Donors, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/individual human semen and plasma from normal healthy donors/product/BioIVT Inc
Average 90 stars, based on 1 article reviews
individual human semen and plasma from normal healthy donors - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
serum samples of 10 healthy controls and 10 gastric cancer patients  (BioIVT Inc)
90
BioIVT Inc serum samples of 10 healthy controls and 10 gastric cancer patients
Nef expression and <t>HIV-1</t> infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.
Serum Samples Of 10 Healthy Controls And 10 Gastric Cancer Patients, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/serum samples of 10 healthy controls and 10 gastric cancer patients/product/BioIVT Inc
Average 90 stars, based on 1 article reviews
serum samples of 10 healthy controls and 10 gastric cancer patients - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
plasma from individual confirmed ad patients (n 5 20) and age-matched human controls (n 5 30) and pooled normal human plasma  (BioIVT Inc)
90
BioIVT Inc plasma from individual confirmed ad patients (n 5 20) and age-matched human controls (n 5 30) and pooled normal human plasma
Nef expression and <t>HIV-1</t> infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.
Plasma From Individual Confirmed Ad Patients (N 5 20) And Age Matched Human Controls (N 5 30) And Pooled Normal Human Plasma, supplied by BioIVT Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasma from individual confirmed ad patients (n 5 20) and age-matched human controls (n 5 30) and pooled normal human plasma/product/BioIVT Inc
Average 90 stars, based on 1 article reviews
plasma from individual confirmed ad patients (n 5 20) and age-matched human controls (n 5 30) and pooled normal human plasma - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier

Image Search Results


Nef expression and HIV-1 infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.

Journal: AIDS Research and Human Retroviruses

Article Title: HIV Type 1 Nef Is Released from Infected Cells in CD45 + Microvesicles and Is Present in the Plasma of HIV-Infected Individuals

doi: 10.1089/aid.2009.0170

Figure Lengend Snippet: Nef expression and HIV-1 infection induce vesicle formation. TEM image of conditioned media from HEK293 cells transfected with (A) Nef expression plasmid or (B) mock-transfected. Conditioned media collected 3 days posttransfection and subjected to ultracentrifugation (400,000 × g). Pellets were fixed, embedded, and stained with 2% uranyl acetate. Samples were visualized using a JEOL 1200 EX transmission microscope. (C) Culture supernatant from HEK293 transfected with a Nef expression plasmid was subjected high-speed differential centrifugation. Supernatants were centrifuged for 10 min at 300 × g, followed by 30 min at 10,000 × g, and then centrifuged for 1 h at 300,000 × g. Nef was detected in vesicles pelleted at 300,000 by immunoblot using anti-Nef monoclonal. S, supernatants; P, pellet.

Article Snippet: Plasma from HIV-1 + /HCV − /HBV − individuals ( n = 10) and HIV − / HCV − /HBV − controls ( n = 10) was obtained from Bioreclamation (Long Island, NY).

Techniques: Expressing, Infection, Transfection, Plasmid Preparation, Staining, Transmission Assay, Microscopy, Centrifugation, Western Blot

Nef vesicles captured using anti-CD45 magnetic bead separation. Supernatants from HIV-1-infected cells were harvested 15 days postinfection and vesicles/virions were concentrated by centrifugation at 200,000 × g for 1 h on a 20% sucrose cushion. The pellet was resuspended in 1 × TNE buffer and subjected to CD45 magnetic bead separation. Each fraction was collected, ultracentrifuged at 400,000 × g for 1 h, and then separated by SDS-PAGE. Fractions (SM = starting material, FT = flow through, W = wash, and E = eluate) were analyzed by Western blot for HIV proteins using anti-HIV sera (upper panel). The singular band present in the eluate was confirmed to be Nef via an immunoblot using an anti-Nef monoclonal antibody (lower panel). Nef+, recombinant Nef protein.

Journal: AIDS Research and Human Retroviruses

Article Title: HIV Type 1 Nef Is Released from Infected Cells in CD45 + Microvesicles and Is Present in the Plasma of HIV-Infected Individuals

doi: 10.1089/aid.2009.0170

Figure Lengend Snippet: Nef vesicles captured using anti-CD45 magnetic bead separation. Supernatants from HIV-1-infected cells were harvested 15 days postinfection and vesicles/virions were concentrated by centrifugation at 200,000 × g for 1 h on a 20% sucrose cushion. The pellet was resuspended in 1 × TNE buffer and subjected to CD45 magnetic bead separation. Each fraction was collected, ultracentrifuged at 400,000 × g for 1 h, and then separated by SDS-PAGE. Fractions (SM = starting material, FT = flow through, W = wash, and E = eluate) were analyzed by Western blot for HIV proteins using anti-HIV sera (upper panel). The singular band present in the eluate was confirmed to be Nef via an immunoblot using an anti-Nef monoclonal antibody (lower panel). Nef+, recombinant Nef protein.

Article Snippet: Plasma from HIV-1 + /HCV − /HBV − individuals ( n = 10) and HIV − / HCV − /HBV − controls ( n = 10) was obtained from Bioreclamation (Long Island, NY).

Techniques: Infection, Centrifugation, SDS Page, Western Blot, Recombinant

Production of CD45+ Nef microvesicles is cell-type dependent. Concentrations of p24 (A) microvesicles and Nef (B) were measured by ELISA in culture supernatants and CD45 captured microvesicles of HIV-1-infected Jurkat T-lymphocytes, THP-1 monocytes, and PMA-treated THP-1 (macrophage-like). T-lymphocytes release the most HIV-1 virus as measured by p24 ELISA whereas macrophage-like THP-1 releases the most Nef microvesicles.

Journal: AIDS Research and Human Retroviruses

Article Title: HIV Type 1 Nef Is Released from Infected Cells in CD45 + Microvesicles and Is Present in the Plasma of HIV-Infected Individuals

doi: 10.1089/aid.2009.0170

Figure Lengend Snippet: Production of CD45+ Nef microvesicles is cell-type dependent. Concentrations of p24 (A) microvesicles and Nef (B) were measured by ELISA in culture supernatants and CD45 captured microvesicles of HIV-1-infected Jurkat T-lymphocytes, THP-1 monocytes, and PMA-treated THP-1 (macrophage-like). T-lymphocytes release the most HIV-1 virus as measured by p24 ELISA whereas macrophage-like THP-1 releases the most Nef microvesicles.

Article Snippet: Plasma from HIV-1 + /HCV − /HBV − individuals ( n = 10) and HIV − / HCV − /HBV − controls ( n = 10) was obtained from Bioreclamation (Long Island, NY).

Techniques: Enzyme-linked Immunosorbent Assay, Infection